2′5′ Obgoadenylate synthetase, an interferon induced enzyme: direct assay methods for the products, 2′5′ ohgoadenylates and 2′5′ co-oligonucleotides

25 : Graphical Induced Structured Input - Output Methods

Such differences may also be responsible for the cause of hereditary disease. Genetic Association Hypothesis testing then is the study to find which SNP’s are causal (or associated) vis-a-vis a hereditary disease. Going site to site we try and associate the genotype with a phenotype. Usually these genetic association mappings are very sparse, (1 in 1000) and may represent basic differences in b...

25 th Enzyme Mechanisms Conference

Competitive protein-binding assay for 25-hydroxycholecalciferol.

1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 5 SD 14 pmol/ml (1 5.2 & 5.6 nglml). 3. Levels of 25-hydroxycholecalciferol...

Assay for plasma 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 by "high-performance" liquid chromatography.

We describe a new, rapid, and simple column-chromatographic procedure for 25-OH vitamin D3 in plasma. The vitamin is extracted by use of Sep-pak C18 (octadecyl alkylated silicic acid), a short factory-packed reversed-phase column, and 25-OH vitamin D2 + 25-OH vitamin D3 fraction is eluted with methanol/water. The 25-OH D2 and 25-OH D3 are then well resolved on a high-resolution 3-micrometer sil...

Pharmacoeconomics 2007; 25 (1): 25-37

1 Health Technology Assessment Unit, Institute for Research in Extramural Medicine, VU University Medical Center, Amsterdam, The Netherlands 2 International Health Foundation, Utrecht, The Netherlands 3 Department of General Practice, Institute for Research in Extramural Medicine, VU University Medical Center, Amsterdam, The Netherlands 4 Institute for Research in Extramural Medicine, VU Univer...